A super-PAC intent on defeating Republican front-runner Donald TrumpDonald John TrumpDems win from coast to coast Falwell after Gillespie loss: 'DC should annex' Northern Virginia Dems see gains in Virginia's House of Delegates MORE has released a new ad that slams him for skipping Thursday’s presidential debate and questions whether he is a real conservative.

The 60-second ad, from Our Principles PAC, accuses Trump of skipping the final GOP debate before the Iowa caucuses because he “can't handle tough questions.”

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“Like why he'd let millions of illegal immigrants stay in America and even supports a pathway to citizenship,” a narrator states.

The ad then cuts to clips of Trump talking about giving immigrants in the country a way to achieve citizenship.

“How do you throw somebody out that's lived in this country for 20 years,” he says in a clip in the ad. “You just can't throw everybody out.”

The ad argues that Trump supports a path to citizenship because he makes “big money off illegal immigrants.”

“Amnesty for illegal immigrants. Big money for himself,” the ad concludes. “Can conservatives trust Donald Trump?”

Our Principles PAC was started by Katie Packer, the former deputy campaign manager for 2012 GOP presidential nominee Mitt Romney.

The group, which started its campaign earlier this week, released a radio ad on Saturday that quizzes listeners on liberal positions taken by Trump in the past, including issues related to gun control and healthcare.

Immigration has been a central theme of Trump’s campaign; he has promised to build a wall along the southern border and make Mexico pay for it.