"Three times they said no to God. That's Patrick Murphy's party. Are those your values?" the ad's text reads.
West has been an outspoken supporter of Israel and has criticized Obama before for not doing enough to protect the country's security.
The description below the advertisement on YouTube says that West "is leading the fight against the radical left's attack on God and Israel," and asks supporters to contribute money to his campaign. The lawmaker earlier issued a release on the platform changes, but has now turned the debacle on the convention floor into a campaign ad.
He faces a tough challenge in Murphy, and the most recent Democratic poll of the race put them neck-and-neck. Democrats believe this is one of the races that they may be able to win to bring them to a majority in the House.
Watch the ad: