The 30-second spot, the DSCC's second in the state, features a woman named Pamela Howze lamenting Allen's positions as clips outlining his various votes flash on the screen.
The ad says he voted against measures to protect women from discrimination and opposed the rights of women who were sexually assaulted in the workplace.
"George Allen is the last thing we need in Washington," Howze says at the end.
Allen is locked in a tight race with former Virginia Gov. Tim KaineTim KaineHispanics on Clinton's VP shortlist could help her win votes The Hill's 12:30 Report Clinton’s 9 most likely VP picks MORE (D) in a race that Republicans consider one of a handful that could bring them control of the Senate.