At least eight of the 24 House Democrats who voted for last month's farm bill are expected to vote against the new measure on Thursday.
The rejection of the revamped House GOP bill means that Republicans will have to carry the bill to passage mostly, if not entirely, on their own.
Rep. Justin Amash (R-Mich.) told The Hill he will vote no but said he believes it will have the votes to clear the lower chamber.
Republicans must minimize defections. If every member votes and all Democrats vote no, GOP leaders can only afford 16 defections.
In June, the first version of the farm bill to hit the House floor failed 195-234. Twenty-four Democrats voted yes while 62 Republicans voted no.
Since then, House Republicans have chopped up the measure, stripping food stamp provisions from the bill.
Democratic Reps. Jim Costa (Calif.), Henry Cuellar (Texas), William Enyart (Ill.), John Garamendi (Calif.), Joe Garcia (Fla.), Collin Peterson (Minn.), Kurt Schrader (Ore.) and Tim Walz (Minn.) voted yes in June. But all eight are planning to vote no or are leaning no this time.
Other Democrats surveyed by The Hill did not comment.