The $30,000 buy represents the first outside spending against McConnell in the GOP primary. The group has endorsed McConnell's Republican challenger, Matt Bevin.
The ad, which will air statewide, hits McConnell's conservative record, highlighting the senator's votes for “amnesty for millions of illegal immigrants” and for the $700 billion Wall Street bailout.
“After 28 years of Mitch McConnell claiming to be a conservative but then supporting big government policies, isn't it time conservatives dumb Mitch McConnell?” the voiceover says.
McConnell released internal polling last week that shows him with a substantial lead over Bevin. According to the survey, McConnell has 68 percent support among Republican primary voters, compared to Bevin's 21 percent.