Rubio has formed a conservative coalition in the Senate with Sens. Mike LeeMike LeeTrouble draining the swamp? Try returning power to the states Congress must act to protect data privacy before courts make surveillance even easier Five tough decisions for the GOP on healthcare MORE (R-Utah), Ted CruzTed CruzThe media couldn't be more blatant in distorting Trump's words on Charlottesville Curtis wins GOP primary for House seat vacated by Jason Chaffetz Kimmel: Let’s make Trump a king so he has no power MORE (R-Texas) and Rand PaulRand PaulCurtis wins GOP primary for House seat vacated by Jason Chaffetz Glimmer of hope in bipartisan criminal justice reform effort Trump barrage stuns McConnell and his allies MORE (R-Ky.) to oppose any short-term spending bill that includes funds for the healthcare law.

Congress will likely need to pass a spending resolution shortly after lawmakers return from the month-long August recess to prevent the government from shutting down.

Rubio said Republicans shouldn't support any funding measure that includes money to implement the healthcare law, including the new insurance exchanges set to launch on Oct 1.

“If this issue isn’t important enough for us to draw a line in the sand on, what issue is?” Rubio said.

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Rubio said one of the reasons he opposes the implementation of ObamaCare is because is it “deeply hurtful to small businesses.”

“The impact this is going to have is not only will people lose their healthcare coverage, they're going to lose their hours,” Rubio said. “This is the real-world impact ObamaCare is having. This is terrible for our country."

The Obama administration has delayed for a year the employer mandate in the healthcare law, which requires businesses with more than 50 full-time employees to provide health insurance.

Republicans say the mandate will cause businesses to lay off workers and reduce hours in order to escape the requirements.