Speaker John Boehner (R-Ohio) and House Majority Leader Eric Cantor (R-Va.) addressed the crowds in 2012.
Each touted the House's efforts to end federal funds for Planned Parenthood as a healthcare provider for low-income women.
"We come today to stand together and voice our belief that innocent life is precious, and the taking of life morally wrong," Cantor said last year. "Our dollars should never be used to take life. It is time to protect conscience rights for those who oppose abortion."
Federal law prohibits taxpayer dollars from supporting abortions. But opponents of Planned Parenthood say any payment to the group subsidizes its abortion services.
The 2012 march began with a rally on the National Mall, near the Smithsonian Castle, and ended in front of the Supreme Court.
The event closed major sections of Constitution and Independence Avenues, as well as streets around D.C.'s Verizon Center, where a prayer vigil was held.
Organizers lamented Thursday that this year's march will not include the event's founder, Nellie Gray, who died in August.
"The March for Life is a demonstration of unity that we need to continue the fight to win over the hearts and minds of all Americans," said Jeanne Monahan, President of the March for Life Education and Defense Fund, in a statement.