The $54 million effort was primarily paid for by ObamaCare's Prevention and Public Health Fund.
One ad featured a woman, Terrie, who smoked up to two packs a day and was diagnosed with oral and throat cancer at age 40. Her larynx was eventually removed and she speaks using an artificial voice box.
Another man, Bill, had his leg amputated due to poor circulation that was exacerbated by smoking. He also suffers from diabetes, blindness and kidney problems.
Officials with the CDC praised the campaign as a cheap way to discourage smoking, one of the leading preventable causes of death in the United States. About 1 in 5 U.S. deaths is connected to cigarette use, the agency said.
Health advocates also touted the campaign's first phase as an "incredible success" and argued that the tobacco industry stands in the way of efforts to quit smoking.
"The impressive results from the first phase of this campaign prove the value in continuing this education effort for years to come," said the American Cancer Society Cancer Action Network in a statement.