"You're closer to the White House than I am," Mica said to Rahall, who did not publicly endorse President Obama's reelection bid.
"I saw all those pictures of you with Obama," Mica continued, referring to 2012 campaign commercials run against Rahall in conservative West Virginia.
Mica triggered laughs when he later said, "I had more."
Rep. Sandy Adams (R-Fla.) ran ads against Mica in their member-versus-member primary featuring Obama thanking the GOP chairman for his role in the passage of a $105 billion transportation bill earlier this year.
But Mica won the primary, and later secured his 11th term in the House. Mica is term-limited as chairman of the Transportation panel.