The ad paid for by billionaire Tom Steyer calls Keystone a "sucker's deal" for the U.S.
The groups want Obama to reject Keystone XL in his State of the Union speech.
Senate's 45 Republicans say it's time for a decision by the president.
The chairman of the House Transportation Committee said Friday that lawmakers in both parties needed to be “educated” on what needs to be included in a new road and transit funding bill.
Speaking at the U.S. Conference of Mayor’s annual conference, Rep. Bill Shuster (R-Pa.) said he needed the help of municipal leaders to convince lawmakers to make compromises that will be necessary to approve a new surface transportation bill.
The report would be the first indication of State's study of the pipeline.
Transportation Secretary Anthony Foxx said Thursday that the projected funding shortfall for road and transit projects this year is a "serious problem" that needs a long-term solution.
Speaking at the U.S. Conference of Mayors' annual meeting, Foxx warned that the Highway Trust Fund running out of money would have ripple effects for cities and other levels of local government.
Oil began flowing through the pipeline's southern leg on Wednesday.
The groups say the oil flows are a "painful example" of Obama's energy policy.
He said State is doing everything it can to "effectively and rapidly" finish pipeline review.
Sen. Landrieu and others met with top Canadian officials to push approval of the pipeline.