The ad does not mention the President Obama's healthcare overhaul, the divisive law that Ryan and GOP presidential nominee Mitt Romney have vowed to repeal in office.
In the new ad, Ryan says his plan for healthcare is to "get insurance and government bureaucrats out of the way and force healthcare providers to compete for our business."
He adds: "That way, we can cut costs, improve quality, and make healthcare accessible for everyone."
Romney and Ryan have come under fire from groups that say the GOP ticket's healthcare policies would raise costs and the number of uninsured Americans, compared to the Affordable Care Act.
The Republican ticket argues that the law represents a "government takeover" of healthcare that will only make the system worse.
"Insurance companies and providers should show us their prices and practices so you can make decisions based on cost and quality," Ryan says in the new TV ad. "You should be in charge of your health, not government or insurance bureaucrats."
If Romney and Ryan lose their race for the White House, but Ryan beats Democratic challenger Rob Zerban in Wisconsin's 1st District, he can return to the House next year.
Sens. John McCainJohn McCainMulvaney vows to give Trump straight talk on entitlements Overnight Defense: McCain grills Trump's budget pick | Dems seek to limit Trump on nukes | Senators weigh new round of base closures Overnight Finance: Trump budget pick on the hot seat | Dems' T infrastructure plan | Deficit to hit 1B in 2019 | Trump meets automakers | Pipelines back on MORE (R-Ariz.) and John KerryJohn KerryPossible US embassy move to Jerusalem exposes facade of two-state negotiations Maryland golf club will extend invitation to Obama, despite objections Obama released 1M to Palestinians in final hours MORE (D-Mass.) did the same after losing their presidential bids in 2008 and 2004, respectively.