The commercial echoes a recent commercial from President Obama's campaign for reelection, which claims the Republicans "turned their back" on auto workers by opposing the bailouts in 2009.
Like the MoveOn commercial released Friday, the Obama campaign's ad singles out Romney and his 2008 op-ed in The New York Times, titled "Let Detroit Go Bankrupt." In the article, Romney called for the automakers to go through a managed bankruptcy.
Democrats have made clear they see the financial turnaround of the auto industry since the federal government lent General Motors and Chrysler more than $80 billion as a political winner for the president in Midwestern states like Michigan, where Republicans are holding a primary next Tuesday.
The auto bailouts began under former President George W. Bush, but Obama has received a large share of the blame for continuing them after taking office in 2009 and for placing conditions on the car companies in exchange for the money.
Romney has defended his opposition to the bailouts, arguing that Obama eventually followed his advice when he made decisions such as forcing General Motors to replace its general manager.
MoveOn said it would air the auto bailout ad on cable stations in Michigan leading up to Tuesday's primary.
Polls have showed Romney locked in a tight race in Michigan with former Pennsylvania Sen. Rick Santorum. Both Romney and Santorum, as well as Republican presidential candidates Newt Gingrich (Ga.) and Rep. Ron Paul (Texas), have said they opposed the auto bailouts.