"No one should ever hit a woman," Beckham tells the camera in the video.
"Not their wife, not their girlfriend, not their date," Lin continues.
The PSA will air this summer on the ESPN Networks, the Fox Sports Networks, MLB Network and NFL Network, according to the White House.
In his renewed personal advocacy since early last year, Biden has directed his comments mainly toward men. Thursday's new PSA reflects that direction, with a variety of all-male athletes chiming in to help publicize Biden's "1 is 2 Many" campaign, which seeks to specifically violence in dating relationships.
"Dating violence hurts all of us," Obama says in the ad. "One is too many."
Biden's campaign responds to statistics that women ages 16 to 24 experience the highest rates of violence at the hands of someone they know, including one in 10 teens reporting physical harm at the hands of a boyfriend or girlfriend and one in five young women sexually assaulted while attending college.
"The worst abuse of power is when a man raises a hand to hurt a woman," Biden says in the ad, urging viewers to "step up, speak out and get help" if they witness a woman in a threatening situation.