The two-and-a-half minute "Yes We Plan" video echoes will.i.am's much-viewed "Yes We Can" video backing Obama's 2008 presidential campaign.
The new film does not mention GOP presidential nominee Mitt Romney or specific policy debates, but alludes to disagreements between Romney and Obama over abortion rights and the mandate that most employers cover birth control for free in their health plans.
The new Planned Parenthood Action Fund clip shows actress Julianne Moore and musicians Mary J. Blige and Q-Tip standing with representatives of the group, backed by music from Bryan-Michael Cox.
"Generation after generation, we have built a better world for our daughters," the speakers say. "We believe that when we make the important decisions about our lives, our heath and our families, we have control over our destinies."
Planned Parenthood's public funding has been a major political issue since Republicans claimed the House in 2010. Defunding the group is an article of faith for abortion-rights opponents, who say taxpayer money need not support a major provider of abortions.
For on Planned Parenthood's Web video, click here.