"Let me just tell you, you've got no business in politics unless you respect the judgment of people and if you run for office you've got to be able to take it, either way winning or losing. And I accept the decision of the voters and I have already offered my sincerest congratulations to Senator-Elect Warren," Brown said.
The exiting senator had to stop the audience from booing at one point, telling the crowd, "no, no, no."
"Listen, she won it fair and square, folks, and she has received the high honor, she has received the high honor of holding the people's seat and may she bring, may she bring that Senate office, may she bring that Senate office great credit just as I set out to do nearly three years ago," he said.
Brown struck a conciliatory and lighthearted tone during his speech, where he fed off the energy of the crowd and profusely thanked his family and supporters.
He joked that the "most difficult part" of losing was that "I now have to break the news to my truck that I'll be taking it home."
Brown has become well known for driving his iconic truck around the state. The senator also poked fun at his 2010 acceptance speech, saying to laughter "just for the record, I'm absolutely not going to say anything about my daughters tonight."
He maintained that he was proud of his service and "independent voice" in the Senate.
"What matters even more is what we achieved in between these two elections. I kept my promise, I kept my promise to you. I kept my promise to you to be that independent voice for Massachusetts and I have never, ever, ever regretted any decision I have made for you. Listen, you all sent me to Washington to be my own man and I'll be returning my own man and for that I am very, very proud, so thank you for that opportunity," he added.