"ObamaCare's coming. It's already causing layoffs. Higher premiums are next. Mitch McConnell saw it coming, leading the fight against ObamaCare," a narrator says in the ad.
"On healthcare, Mitch McConnell stood with Kentuckians on day one," the narrator adds.
The ad, launched by Kentucky Opportunity Coalition, is backed by a $109,000 buy and will run for a week in Lexington and Louisville.
Many provisions of the health care reform law are slated to go into effect in 2014, right as voters tune in to politics in time for House and Senate elections.
Indeed, Democrats have expressed concern that a botched implementation of ObamaCare could cost them seats in both chambers.
In Kentucky, however, Democrats have yet to front a candidate to run against McConnell, and he's thus far run only positive advertisements touting his own connection to the state and featuring his wife decrying liberal attacks.
Democrats are still watching Secretary of State Alison Lundergan Grimes, their best chance at taking the seat, to see whether she decides to enter the race.
--This piece was updated at 1:40 p.m. to reflect the group's status as a nonprofit.