The video features clips of news anchors discussing the across-the-board, automatic spending cuts that went into effect on March 1, as well as comments by Boehner.
Pelosi's spokesman, Drew Hammil, said the video is aimed at comparing Boehner's views on the sequester from a 2011 CBS interview and a recent press conference.
At a press conference last Friday, Boehner said "It was a negotiation. I didn't like it anymore than anyone else liked it."
Also on Wednesday, the Republican National Committee (RNC) unveiled its own Web ad accusing Democratic leaders of failing to “admit” the nation has a spending “problem.”
“Barack Obama, Nancy Pelosi and Congressional Democrats simply do not believe that we have a spending problem," said RNC Chairman Reince Priebus in a statement. “Every day, families across this country are forced to watch their spending and live within their means. There’s no reason that shouldn’t be the case in Washington."