The Associated Press snapped the shot at Cruisers Diner in Seaman, Ohio on Sunday.
A White House pool report says the bikers may be part of a group called the "Shaddowmen." No details were available on their real names or what discussion led to the lap incident.
Some observers were quick to joke that Biden is starting to resemble the working-class parody Biden seen in The Onion.
Earlier in the diner visit, Biden made banter with biker Jeff Cook.
"Can I borrow one of your bikes? They don't let me ride anymore."
"Probably not," Cook said.
"Probably not," the Veep responded, according to the pool report.