The Lincoln Project, an anti-Trump GOP super PAC, released an ad Tuesday jabbing President TrumpDonald John TrumpNew Bob Woodward book will include details of 25 personal letters between Trump and Kim Jong Un On The Money: Pelosi, Mnuchin talk but make no progress on ending stalemate | Trump grabs 'third rail' of politics with payroll tax pause | Trump uses racist tropes to pitch fair housing repeal to 'suburban housewife' Biden commemorates anniversary of Charlottesville 'Unite the Right' rally: 'We are in a battle for the soul of our nation' MORE over a testy interview with Fox News host Chris Wallace that aired over the weekend.

The ad begins with an image of the White House and the caption "Trumpfeld," which is modeled after the logo for "Seinfeld," along with music similar to the hit '90s sitcom's signature theme song.

The video plays a laugh track over several of Trump’s comments, including his repeated denial of several Fox News polls.

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The first poll showed that 49 percent of surveyed voters said they would vote for former Vice President Joe BidenJoe BidenRon Johnson signals some GOP senators concerned about his Obama-era probes On The Money: Pelosi, Mnuchin talk but make no progress on ending stalemate | Trump grabs 'third rail' of politics with payroll tax pause | Trump uses racist tropes to pitch fair housing repeal to 'suburban housewife' Biden commemorates anniversary of Charlottesville 'Unite the Right' rally: 'We are in a battle for the soul of our nation' MORE, the presumptive Democratic nominee, if the election were held today, compared to 41 percent who said the same about Trump.

“First of all, I’m not losing,” the president told Wallace, with the group's ad then including audio of a crowd laughing.

“Those are fake polls,” Trump claimed in a later clip included in the ad, followed by more added laughter.

The president in the interview also denied a poll from the outlet showing voters trusting Biden more to manage the ongoing pandemic, race relations in the U.S. and the economy.

“I have other polls that put me leading,” Trump said.

He also denied a Fox News poll showing that 51 percent of survey respondents said Trump does not have the “mental soundness to serve effectively as president.” Forty-three percent of those polled disagreed.

Forty-seven percent of survey respondents said Biden has the “mental soundness to serve effectively as president,” compared to 39 percent who disagreed.

“Let’s take a test,” the president told Wallace.

“Joe and I will take a test. Let him take the same test that I took,” Trump continued over the sound of laughs in the Lincoln Project's ad.

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Trump had frequently challenged Biden to take a cognitive test. Wallace told Trump during their interview that he had also taken the test that the president claimed earlier this month that he had “aced.”

“It’s not the hardest test. They have a picture, and it says 'What’s that?' and it’s an elephant,” Wallace said.

The president claimed the test “gets very hard, the last five questions.”

“Don’t you believe America deserves a president who doesn’t brag he can spot an elephant?” the Lincoln Project's ad asks.

Trump campaign spokesperson Erin Perrine pushed back on the latest ad, saying in a statement to The Hill, “This is the swamp – yet again – trying to take down the duly elected President of the United States." 

"President Trump is the leader of a united Republican Party where he has earned 94% of Republican votes during the primaries – something any former president of any party could only dream of," Perrine said. 

The Lincoln Project said last week that it raised $16.8 million in the second quarter of 2020 as it ratchets up efforts targeting Trump ahead of the November election.

Updated: 9:15 p.m.