Fox News host Megyn Kelly repeatedly challenged Donald TrumpDonald TrumpVirginia GOP gubernatorial nominee acknowledges Biden was 'legitimately' elected Biden meets with DACA recipients on immigration reform Overnight Health Care: States begin lifting mask mandates after new CDC guidance | Walmart, Trader Joe's will no longer require customers to wear masks | CDC finds Pfizer, Moderna vaccines 94 percent effective in health workers MORE's campaign manager, Kellyanne Conway, about controversies involving the Republican presidential nominee and his treatment of women in a testy exchange on Wednesday night’s “Kelly File.”

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Kelly, who has been on the receiving end of more than a couple of Trump's comments herself, refused to accept Conway’s claim that the billionaire has made just a “couple of comments over 25 years."

“You know that's not true. You know,” Kelly fired back. "You know he has repeatedly made comments about women, about their looks, about their size, their weight. Even in this campaign, talking about [former GOP presidential candidate] Carly Fiorina's face, retweeting a negative picture about Heidi Cruz's face, criticizing Hillary ClintonHillary Diane Rodham ClintonDemocrats say it's up to GOP to stop Trump 2024 Hillary Clinton to speak at Asian Americans and Pacific Islanders summit More than half of eligible Latinos voted in 2020, setting record MORE and her 'look.' And, Kellyanne, this is an issue for him, is it not?”

Conway pushed back by criticizing Clinton, the Democratic presidential nominee, for spending millions on ads hitting Trump on his treatment of women instead of putting forward her plan for America.

"I’m just wondering why she’s spending tens of millions of dollars in negative ads and negative mail pieces and never putting together an aspirational, positive vision,” Conway said.

“Because she’s killing him with women and she sees an advantage there and she’s trying to exploit it,” Kelly replied, turning to Trump's most recent comments about former Miss Universe Alicia Machado. “And my question to you is whether he need to come up with a better answer than telling ‘Fox and Friends,’ 'Well, she gained a bunch of weight when she won Miss Universe and she was a real problem.' "